Description
Clinical demand for vitamin D measurement is increasing as additional biological effects are postulated for vitamin D, and in light of the significant level of deficiency that has been found in many populations.The measurement of vitamin D is complicated by the multiple metabolites present in serum and the various assays available.
This review briefly explores the main issues regarding 25-hydroxyvitamin D measurement and the
problems encountered. In particular the measurement of 25-hydroxyvitamin D2 and D3, the use of exogenously spiked calibrators and the role of vitamin D binding protein are discussed. The advantages and disadvantages of the main methods used are summarised especially automated immunoassays and liquid chromatography mass spectrometry.
